GROEL and GST
d-fesque at nimr.mrc.ac.uk
Tue Jul 8 13:43:58 EST 1997
about your question!
I am coexpressing GroEL and GroES with a GST fusion protein.
The GroEL seems to copurify with my desired GST fusion protein on the
glutathione sepharose 4B column. Has anyone experienced this?
University of Texas at Austin
I think you will have problem to get rid of the chaperone protein while
keeping your GST protein soluble. nevertheless, I have seen in the GST
booklet from pharmacia that you can get rid of DNAK chaperone which is
very often associated with GST with a cocktail of ATP/MgCl. I remenber a
PNAS paper a few years ago (one of the author is stieger) describing the
coexpression of a kinase with GROEL, using a special buffer they succeed
to get their protein soluble without GROEL/GROES! have a look in
IF you are successful pleased tell me
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