Single-stranded nicks in plasmids

Karl Fischer tyr-2 at bones.biochem.ualberta.ca
Tue Jul 8 13:41:29 EST 1997


> On 7 Jul 1997 17:02:23 -0700,
> Kevin Williamson (ksw at novadx.com) wrote:
> 
> >I need to know how to introduce a nick into one strand of a ds-plasmid 
> >so that I can use ExonucleaseIII to remove the nicked strand- 
> >effectively converting a double-stranded plasmid library into a single 
> >stranded one for hybridisation.

gene II product/exo III combo should do the trick a la the BRL/Gibco
GeneTrapper (tm) cDNA positive selection system.

No affilliations.

Karl the hepB guy

-- 
Karl Fischer
tyr-2 at bones.biochem.ualberta.ca





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