HPLC Column Choice?

Frederick W. Hyde fredhyde at citilink.com
Thu Jul 10 00:31:47 EST 1997


Christian Wold wrote:
> 
> Im trying to optimise a separation of ethanol extracted components from
> cooked beef samples. I am using reverse phase, gradient elution
> (acetonitrile/water) and have tried a range of C18 columns with little
> success. The molecules are quite polar and are eluting quite early in the
> run, my peaks are tending to coelute and tail.

Are the compounds you are assaying volatile at all?

Fred



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