Semi-quantitative RT-PCR control

huang zhong zhong.huang at student.kuleuven.ac.be
Wed Jul 9 18:47:24 EST 1997


Alun Kirby wrote:
> I was going to use beta-actin but have been told that this varies
> between tissues. Any info on which primers to use and for what template
> would be gratefully appreciated. GAPDH perhaps?
> 
> Thanks.....Alun.
> 
> e-mail to akirby at eastman.ucl.ac.ukYou can try GAPDH as internal standard. It's simple and easy to 
operation. You also can try external sequence which will competete with 
the same priming sites with your target primers (so called competative 
PCR or MIMIC PCR).MIMIC PCR kit is available from clonetech.
-- 
--------------------------------------K.U.Leuven---------------------
HUANG Zhong
Lab of Gut Hormone        => http://urc1.cc.kuleuven.ac.be/~m9631766/
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