electroporation of rodent cells

Deb Britt Deborah_Britt at brown.edu
Thu Jul 10 13:23:08 EST 1997

I have been trying to get a rather large (12 kb) plasmid into a rat cell
line using lipofectamine (and calcium phosphate one time), but have not
had much luck getting gene expression.  I want to try electroporation, but
am not sure of the parameters to use.  How does one manipulate
electroporation conditions to get large plasmids into the cell, and to
encourage multiple copies?  Thanks for the info.

Deborah Britt, Ph.D.
Department of Medical Oncology
Rhode Island Hospital

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