COS cells/MMT I promoter

Tom Vink tvink at
Thu Jul 10 05:27:34 EST 1997

Andrea Marie Peier <ap691301 at> wrote:

>Does anyone know if the mouse metallothionein I promoter works well in
>COS cells. My selectanle marker gene (neo) is driven by this kind of
>promoter. I've been having problems with my transfections and am
>wondering if this promoter functions OK in this cell type.
>Any answer or references is appreciated.

We have used the PNUT vector to express proteins in cos cells. This
expression is driven by the mmti promotor and works fine in our hands.
Be sure to inactivate your sv40 ori when stable transfecting Cos cells
though .

Greetings Tom

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