membrance associated protein

FC bb936 at
Thu Jul 10 10:39:00 EST 1997

I am going to try a small project which to measure 
Fas expression in response to treatment.  I will 
extract proteins from cells and detect Fas level by 
immunoblot.  I read some articles and this is the 
way to do it.  However, I was suggested that I have 
to make sure the Fas is extracted since Fas is a 
membrance associated protien, I may lost majority of 
Fas by conventional extraction buffer.  Anyone has 
any ideal what I can do to harvest membrane 
associated protein, or suggest some refference I can 
read.  Thank you very much

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