Adding IPTG and X-gal to LB plates-puzzle.

Alex Dobrovic adobrovic at
Fri Jul 11 04:41:06 EST 1997

Why is it that more IPTG and X-gal are added when one spreads them on
previously made LB antibiotic plates than when IPTG and X-gal are included
in the LB agar before pouring? Is it an old mistake that everyone
replicates or is there a reason?


Alexander Dobrovic, Ph. D.
Chief Scientist
Department of Haematology-Oncology
The Queen Elizabeth Hospital, Woodville, SA 5011, Australia

Affiliate Senior Lecturer
Department of Medicine, University of Adelaide

Tel 61-8-8222 6884 Fax 61-8-8222 6046

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