Electroporate the THP-1 cell line.

jh7 at acsu.buffalo.edu jh7 at acsu.buffalo.edu
Fri Jul 11 13:33:43 EST 1997


Hi there:

Can anyone provide electroporation protocol for the THP-1 cell line?

We are using the BTX Electro Cell Manipulator 600 and 2 mm gap cuvette.
I would prefer 10 million cells for each sample. 

We tried to find the condition these days, but it seems the cells are very
difficult to recover after the electroporation. However, under mild
condition, transfection efficiency is too low. 

If anyone happen to have the successful experience, could you reply?

Thanks in advance!

 





***************************************
Yuan Shen
Dept. of Microbiology/Immunology
Roswell Park Cancer Institute
State University of New York at Buffalo




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