Dr. Duncan Clark
duncan at genesys.demon.co.uk
Fri Jul 11 03:43:25 EST 1997
In article <33C50950.1059 at uvic.ca>, "Kris W." <kwilde at uvic.ca> writes
>The other day I was digesting a PCR-Script vector with EcoRI and I found
>that it cut at two sites when I was only expecting it to cut at one. I
>normally leave the digestion for an hour or so but this time I left it
>for 3 hours. I re-did the digestion for a half hour and found that
>there was only a single cut. After looking at the sequence and the
>sizes of the fractions caused by the digestion I concluded that EcoRI
>(gaattc) cuts at aaattc when left for too long. Similar sites such as
>gaattg were not cut.
>Has anyone else noticed this or similar incidences with other
Classic star activity. Check out the appendix in a NEB catalogue.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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