Adding IPTG and X-gal to LB plates-puzzle.

Richard L lm11 at gpu.srv.ualberta.ca
Fri Jul 11 19:31:33 EST 1997


Alex Dobrovic <adobrovic at medicine.adelaide.edu.au> wrote:
: Why is it that more IPTG and X-gal are added when one spreads them on
: previously made LB antibiotic plates than when IPTG and X-gal are included
: in the LB agar before pouring? Is it an old mistake that everyone
: replicates or is there a reason?

: Tel 61-8-8222 6884 Fax 61-8-8222 6046

Spreading Xgal and IPTG on a previously made plate is mainly to save
money, because Xgal is too expensive. Probaly you are using too much Xgal.
Try to use less.

Richard





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