EcoRI problem

Deb Schuback schuback at helix.mgh.harvard.edu
Fri Jul 11 16:39:58 EST 1997


I think that the problem was caused by star activity. The longer you left
the digestion in the H2O bath, the more water evaporated causing a
concentration and star activity
Debs



On Thu, 10 Jul 1997, Kris W. wrote:

> The other day I was digesting a PCR-Script vector with EcoRI and I found
> that it cut at two sites when I was only expecting it to cut at one.  I


Schuback at helix.mgh.harvard.edu
Lab Manager/Senior Tech
Massachusetts General Hospital
Molecular Neurogenetics





More information about the Methods mailing list