EcoRI problem

Deb Schuback schuback at
Fri Jul 11 16:39:58 EST 1997

I think that the problem was caused by star activity. The longer you left
the digestion in the H2O bath, the more water evaporated causing a
concentration and star activity

On Thu, 10 Jul 1997, Kris W. wrote:

> The other day I was digesting a PCR-Script vector with EcoRI and I found
> that it cut at two sites when I was only expecting it to cut at one.  I

Schuback at
Lab Manager/Senior Tech
Massachusetts General Hospital
Molecular Neurogenetics

More information about the Methods mailing list