Digitonin solubilization

John Richard Seavitt jrseavit at artsci.wustl.edu
Sat Jul 12 16:54:31 EST 1997



On Sat, 12 Jul 1997, Andrey S. Shaw wrote:

> This is typical for digitonin.  Boiling and then cooling and then
> filtering is standard.  We have been buying it from WAKO and this stuff
> all goes into solution.  Apparently, if you take the solution, after
> filtration and then dry it down, you then have the "soluble" fraction
> and can then use this.  
> -- 
> Andrey S. Shaw
> 
 
We get similarly good results with the digitonin from Gallard and
Schlessinger (it's sold as a 99% form, rather than Sigma's 50% form).

John Seavitt




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