amplification of cyp2d6

bentley atchison bentleya at vifp.monash.edu.au
Sun Jul 13 22:40:25 EST 1997


Re: amplifiying CYP2D6 gene

This is difficult to amplify and needs pure DNA (phenol purified).  We
use 1.2 mM Mg in the PCR but this will depend on other components you
use. 0.8 mM sounds too low if the dNTP concentration is 200 microM each.

Bentley Atchison




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