PCR 32-P labeling

Brett Beitzel bbeitzel at jeeves.ucsd.edu
Mon Jul 14 17:15:50 EST 1997


Anyone have a tried and true protocol fo incorporating 32-P into probes
via PCR.  I've been trying to random-prime label a 100 bp fragment, and my
specific activity has been low.  I'm interested in trying PCR labeling to
increase the specific activity of my probes.

Any thoughts, suggestions?

Thanks,

Brett Beitzel
Infectious Disease Lab
Salk Institute
bbeitzel at jeeves.ucsd.edu



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