lysis buffer / protein concentration

Mark Meininghaus Mark.Meininghaus at
Mon Jul 14 10:50:17 EST 1997

i have the problem that i want to mesure the protein concentration of a
whole cell lysate of different human cell lines to compare the protein
expression after transient transfection of plasmids with my protein of
interest. the lysis buffers i know, all interfere with the assay i used:

bradford and smith (BCA) dont like detergents
lowry doesnt like reducing agents

my question:
does anybody know a good lysis buffer that i can use for making whole
cell lysates and that doesnt interfere with the standard protein assays
(bradford or BCA)?

thanks for help


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