Catalase assays???

Robert W. St. George Fisher, IV fisher at
Mon Jul 14 18:57:20 EST 1997

I'm trying to perform enzymatic assays for antioxidant enzymes like
catalase, superoxide dismutase, and glutathione peroxidase in human 
cell lines.  Would anyone that has had experience in applying these
assays to tissue culture cells care to help me?  I'm wondering what
disruption protocol would be best, how many cells and/or amount of
protein to use, do i clear the lysates, etc.

Thank you.
Robert Fisher
fisher at

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