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Hartley, Jim JHartley at lifetech.com
Mon Jul 14 12:55:04 EST 1997


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I chanced upon a staining trick: Stain in 10 mg / liter Coomassie R250
in 10 % methanol, 10 % acetic acid.  This is much  (greater than 100
fold) more dilute than the usual Coomassie concentration.  With hot
(heated in a microwave) solution, faint bands are visible in 5 minutes,
easily visible in 30 minutes.  After 16 - 48 hours the bands are as dark
as conventional protocols, without any solution changes or destaining.

Jim Hartley

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