lysis buffer / protein concentration
Richard J. Dudley
rdudley at nrc.uab.edu
Tue Jul 15 08:59:59 EST 1997
Have you looked at the BCA assay? Sigma or Pierc can supply you with a
list of compatible reagents. There's also and analytical biochemistry
paper by Smith, et al. testing the limits of the assay (150: 76-85,
Perhaps you could look into a protein precipitation method also (soch as
Wessel and Flugge, Analytical Biochemistry 138: 141-143 1984).
In addition, a number of companies sell resins that remove detergents.
Try Bio-Rad, Pierce, and Calbiochem.
I have used the Wessel and Flugge method when I was in your position,
and it wroks very well. Sometimes you need a little urea or guanidine
to resuspend the protein after it's precipitated, though.
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* Argue not with dragons, for thou art crunchy and go well with brie. *
Richard J. Dudley (rdudley at nrc.uab.edu)
Department of Neurobiology
University of Alabama School of Medicine
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