painful gel loading???!!!!!!

brendon priceb at biochem.unp.ac.za
Tue Jul 15 01:55:11 EST 1997


Hi
Do you have glycerol in your loading buffer, since that should allow the
DNa to sediment?

Kirill Kashkin <kirill at win.pop.online.ru> wrote in article
<5qebll$b21$2 at news.sovam.com>...
> xudong huang <xudong.huang at endo.mas.lu.se> wrote:
> 
> >This is a multi-part message in MIME format.
> 
> >--------------6860717F2D0C
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> 
> >Hello,
> 
> >I have a painful problem about agarose gel loading. The DNA samples do
> >not stay at the bottom of wells, intead, they float out of wells and
> >diffuse into the buffer. The loading buffer I use is 6XBPB, the gel
> >running buffer is 1X TBE. It is not because of air bubbles or oil in the
> >samples. Could you let me know what is going on? Thank you.
> 
> Hi! The simplest thought I've got: may be Your DNA is not good dried
> from ethanol? (it is less dense than water). Or smth else.
> 
> 



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