RNA primer extension

Sami K J Kukkonen skjkukko at cc.helsinki.fi
Mon Jul 14 08:50:46 EST 1997


Has anyone used an RNA polymerase to extend an RNA primer annealed to a
DNA template? 
Which RNA polymerases should work? 
If there are no T7 or SP6 promoters present in the DNA template, would
those RNA polymerases be able to extend the RNA primer?

I'd be very grateful if anyone could help me with the above questions.
--
Sami



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