HELP!!! DNAse treating human adipose RNA
graham at biodec.wustl.edu
Thu Jul 17 10:54:18 EST 1997
Try "washing" the extracted RNA several times by resuspending in
guanidiuium lysis buffer and alcohol reprecipitation. After two or three
rounds, incubate an aliquot of the sample in your DNAse buffer at 37C
for about 30 minutes and compare it on a fomraldehyde get to an
untreated sample (eg. resuspended in GuThCy or loading buffer only).
You need to establish that your RNA is stable in the DNase treatment.
(Store all RNAs as alcohol precipitated aliquots -by the way, and don't
heat RNA over 70C, particularily if you have not chelated metal ions
with EDTA first -and even if you have. :)
J. Graham PhD
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