RNAse T1

zinc zinc at zifi.genetics.utah.edu
Thu Jul 17 23:19:37 EST 1997


-----BEGIN PGP SIGNED MESSAGE-----

Matt,

we've seen a little of this sort of thing in our lab. it seems to be
an artifact of the transcription. do your RNAs start with three Gs? T7
often stutters with those and the result is a non-homogeneous pool of
RNAs that give 'shadow' bands.

good luck,

- -pjf


Matt Thomas <Thomas at molbio.uoregon.edu> writes:

> Does anyone know of conditions where RNase T1 will cut other then after
> a G?  IT is advertised that it cuts only after G's but I have some
> strange digestion patternes which suggest sometimes it cuts weird.  The
> DNA sequence that I make the RNA is fine, and I'm checking the RNA
> directly with primer extension, but I want to rule out that it is the T1
> that is doing strange things.
> 
> Thanks
> Matt Thomas

- -- 
"Those that give up essential liberty to obtain a little temporary
safety deserve neither liberty nor safety." -- Benjamin Franklin (1773)
		   finger zinc-pgp at zifi for PGP key
zifi runs LINUX 2.0.30  -=-=-=WEB=-=-=->  http://zifi.genetics.utah.edu

-----BEGIN PGP SIGNATURE-----
Version: 2.6.2
Comment: Processed by Mailcrypt 3.4, an Emacs/PGP interface

iQCVAwUBM87u0U3Qo/lG0AH5AQFLfQP+OWr+ca+cD5xM1gwX89unatzqpFQlKRA/
LaaURmHoYYuspN3s2R82nBb5GKzmx5z+Q8MJqGjRgOnzdTcq3hckk9Xoe1k5bN4R
+4kuASyhlwxrMzSSjZ4bGQVrxU+j8EnWVZNGNclcCOIfCZ1ChxQP6yFWFpE7czSt
WfbCVmCwvvE=
=JmzV
-----END PGP SIGNATURE-----



More information about the Methods mailing list