Stable transfection of U937 cells

Kevin Mulcahy K.Mulcahy at sheffield.ac.uk
Fri Jul 18 06:39:02 EST 1997


Hi,

has anyone attempted to create stable transfectants of the pre-monocytic 
cell line U937? If so, could you let me know what promoter you used for 
driving the transcription of your insert cDNA and what vector you have 
used for the transfection. Just out of curiosity, if you used G418 as 
the selecting antibiotic, what concentration did you use?



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