GST-fusion

[ilyas m khan]

In article <33C15803.3F30 at mail.utexas.edu>, Daniel Hirschhorn
<d.hirschhorn at mail.utexas.edu> wrote:

> I am coexpressing GroEL and GroES with a GST fusion protein.  
> The GroEL seems to copurify with my desired GST fusion protein on the
> glutathione sepharose 4B column.  Has anyone experienced this?
> Any suggestions?
> 
> Kelley Ruud
> University of Texas at Austin
> kruud at mail.utexas.edu

The answer to your dilemma can be found in Trends in Biochemical Sciences,
January 1997 issue pages 33-34. 


                                          hope this helps

                                             ilyas