Best T/A cloning Taq

Martin Cann mjcann at MED.CORNELL.EDU
Fri Jul 18 11:29:19 EST 1997


I am currently using Clontech KlenTaq to do degenerate PCRs on cDNA and
a subsequent Perkin Elmer Taq secondary PCR before T/A cloning
(Invitrogen).

Can anyone recommend a really good A adding hot start Taq that will do
everything that KlenTaq does for my PCRs?  I have tried AmpliTaq Gold
but have found it to be not nearly as good.

On the other hand what is the best blunt end cloning kit that people
have used?

Many thanks.

Martin

mjcann at med.cornell.edu



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