direct sequencing of PCR products
ogandril at cri.ens-lyon.fr
Thu Jul 17 03:33:29 EST 1997
We routinely sequence PCR products directly after removal of PCR primers
and we are using for the sequence reaction one of the primers that
served for the PCR. And it works (We use Wizard PCR prep from Promega to
purify the PCR product, NO link with promega, any other resins might
Hope this helps
J Atkinson wrote:
> Any hints for successful sequencing of PCR products?
> I want to sequence a 100bp PCR product without cloning. I have been
> told a method exists which uses DMSO in all solutions, anyone know
> anymore. How small can the primer be? Any tips to get sequence as
> close to the primer as possible.
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