direct sequencing of PCR products

Baylor Molecular Genetics Lab Steven_Burghart at baylor.edu
Sat Jul 19 13:24:35 EST 1997


In article <33CDD8D9.419D at cri.ens-lyon.fr>, Olivier.Gandrillon at ens-lyon.fr
wrote:

> Hi 
> 
> We routinely sequence PCR products directly after removal of PCR primers 
> and we are using for the sequence reaction one of the primers that 
> served for the PCR. And it works (We use Wizard PCR prep from Promega to 
> purify the PCR product, NO link with promega, any other resins might 
> work...)
> 
> Hope this helps
> 
> Olivier

Hi Olivier

We are wondering if there any specific protocol for direct PCR sequencing.
We will be appreciate for any references or your protocol.
Yerlan

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