inverse PCR

Sean Rollins rollins.24 at postbox.acs.ohio-state.edu
Sat Jul 19 10:34:17 EST 1997


It is a method to look at unknown sequence adjacent to a known region.  DNA 
can digested with a RE within your known sequence and religated to unknown 
sites to form closed circular molecules.  PCR is used with two oligos 
containing 3' ends facing away from the 5' ends (the opposite way of normal 
PCR).  If your lucky and have chosen a RE that generates a reasonable size 
circular DNA you can amplify neighboring sequence for cloning or 
manipulation.

        	3'-----------------------------5'  NNNNNNNNNNNNNN
	NNNNNNNNNNNNNN  5'----------------------------3'  
 



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