OD260/280 > 2 !?

John E. Blume jblume at ix.netcom.com
Sun Jul 20 10:12:02 EST 1997


In article <33D14E0C.5AA8 at pop.pitt.edu>, akhst7+ at pitt.edu wrote:

> Dear all, 
> 
> Is it possible to get ratio of OD260/280 greater than 2 in total RNA
> prep ?

I believe it's possible to get ratios greater than 2 if contaminating
chaotropic salts remain in the prep. Check the efficacy of your pptn/wash
steps.

John Blume

-- 
John Blume
jblume at ix.netcom.com



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