library screening

Christie Blackman cfb7 at
Tue Jul 22 15:17:08 EST 1997

Oops...forgot to change the sender's name to Christie Blackman; email replies 
to cfb7 at, please.  

Thanks, Christie

In article <mkc125.34.33D51126 at> mkc125 at (Melissa Callahan) 
writes:>From: mkc125 at (Melissa Callahan)
>Subject: library screening
>Date: Tue, 22 Jul 1997 19:59:35 GMT

>I'm wondering:  how does one know when they have saturated a screen?  
>Specifically, I'm using a degenerate PCR strategy to clone new members of a 
>gene subfamily; how do I know when a source of cDNA (excised from Lambda Zap 
>II library) is no longer going to yield new clones?  Or:  how do I 
>statistically predict when a source of RNA is no longer going to yield new 
>clones by RT-PCR?  

>Thanks, Christie

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