cfb7 at psu.edu
Tue Jul 22 15:17:08 EST 1997
Oops...forgot to change the sender's name to Christie Blackman; email replies
to cfb7 at psu.edu, please.
In article <mkc125.34.33D51126 at psu.edu> mkc125 at psu.edu (Melissa Callahan)
writes:>From: mkc125 at psu.edu (Melissa Callahan)
>Subject: library screening
>Date: Tue, 22 Jul 1997 19:59:35 GMT
>I'm wondering: how does one know when they have saturated a screen?
>Specifically, I'm using a degenerate PCR strategy to clone new members of a
>gene subfamily; how do I know when a source of cDNA (excised from Lambda Zap
>II library) is no longer going to yield new clones? Or: how do I
>statistically predict when a source of RNA is no longer going to yield new
>clones by RT-PCR?
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