library screening

Melissa Callahan mkc125 at
Tue Jul 22 14:59:35 EST 1997

I'm wondering:  how does one know when they have saturated a screen?  
Specifically, I'm using a degenerate PCR strategy to clone new members of a 
gene subfamily; how do I know when a source of cDNA (excised from Lambda Zap 
II library) is no longer going to yield new clones?  Or:  how do I 
statistically predict when a source of RNA is no longer going to yield new 
clones by RT-PCR?  

Thanks, Christie

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