mkc125 at psu.edu
Tue Jul 22 14:59:35 EST 1997
I'm wondering: how does one know when they have saturated a screen?
Specifically, I'm using a degenerate PCR strategy to clone new members of a
gene subfamily; how do I know when a source of cDNA (excised from Lambda Zap
II library) is no longer going to yield new clones? Or: how do I
statistically predict when a source of RNA is no longer going to yield new
clones by RT-PCR?
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