DNA purifications

Kristina Palomba kjpalo at ma.ultranet.com
Tue Jul 22 16:43:55 EST 1997


On 3 Jul 1997 17:55:26 GMT, D. KIM <dkim at NMSU.Edu> wrote:

>Richard M L <lm11 at gpu.srv.ualberta.ca> wrote:
>: Dwight M. Nance <dwight at dwight.path.umanitoba.ca> wrote:
>: : I would like to purify DNA fragements from agarose gels (normal agarose)
>: : that range from 200-600bp in length. Does anyone have suggestions as to
>: : which kits work the best
>
>: : JM
>
>: Kit is not necessary, a small tube called SPIN-X is enough. Many
>: manufacturers provide this kind of tube, what you do is:
>
>An alternative is to use an aerosol barrier pipet tip.  Use the cheap ones
>that are not "blocking".  Place the gel slice in the top of the tip, above
>the barrier, and then place the tip in an open Eppendorf tube.  Spin
>gently for a minute (A hard spin bends the end of the tip, sometimes
>closing the opening.).  Do what you will with the eluate.
>
>I often freeze the gel slice in the tip, then spin through. I do not know
>if this improves yield, but it is a habit from my "freeze-squeeze" days.
>
>Daniel Kim
>
Which aerosol tips do you use?



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