Northern problems

H. Yim hyim at u.washington.edu
Thu Jul 24 11:26:18 EST 1997


Could you please include a protocol for adding tRNA or at least give us
the amount of tRNA you need to add to the prehyb? hyb? solution.  Thanks,
Harry Yim

On 22 Jul 1997, J. Hendriks wrote:

> 
> 
> Laboratoire RCMS <diederic at cu.lu> wrote in artikel <33CF3D04.6B69 at cu.lu>...
> > Hello,
> > 
> > Our random primed probes (Gibco kit) seem to hybridize to the ribosomal
> > subunits rather then to any mRNA.
> > 
> > What could be the problem ?
> > 
> > Any answer is appreciated.
> > 
> > Thanks
> > 
> > Marc
> > diederic at cu.lu
> > 
> We have had the same problems. We added some yeast tRNA and that solved the
> problem. Perhaps the problem exists because the stringency is not high
> enough. I don't know it, but our solution worked. By adding tRNA you block
> the aspecific binding-sites like the 28 and 18 S ribosomal RNA's. Good
> luck!
> Brenda Hendriks
> b.hendriks at wkz.azu.nl
> 
> 




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