hyim at u.washington.edu
Thu Jul 24 11:26:18 EST 1997
Could you please include a protocol for adding tRNA or at least give us
the amount of tRNA you need to add to the prehyb? hyb? solution. Thanks,
On 22 Jul 1997, J. Hendriks wrote:
> Laboratoire RCMS <diederic at cu.lu> wrote in artikel <33CF3D04.6B69 at cu.lu>...
> > Hello,
> > Our random primed probes (Gibco kit) seem to hybridize to the ribosomal
> > subunits rather then to any mRNA.
> > What could be the problem ?
> > Any answer is appreciated.
> > Thanks
> > Marc
> > diederic at cu.lu
> We have had the same problems. We added some yeast tRNA and that solved the
> problem. Perhaps the problem exists because the stringency is not high
> enough. I don't know it, but our solution worked. By adding tRNA you block
> the aspecific binding-sites like the 28 and 18 S ribosomal RNA's. Good
> Brenda Hendriks
> b.hendriks at wkz.azu.nl
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