antisense oligo control
Mr. G. Morley
gmorley at hgmp.mrc.ac.uk
Thu Jul 24 11:09:16 EST 1997
>Hi folks --
> I am looking for an assay or a positive control experiment which
>would allow me to ascertain whether antisense oligos were being taken up
>by my cells. I know one possibility would be to look for the protein
>and see whether or not its expression is inhibited, but I do not have
>any antibodies to any of the proteins I want to inhibit.
> Thanks all,
>Harry.Witchel at Bristol.ac.uk
Hmmmm.. what about using a control oligo that will kill cells if it
is expressed.. perhaps to some essential house keeping gene.
ALthough you obviously could not use this oligo in a co-infection
with your desired oligo you could at least see if your cells are
capable of taking up oligos.... not sure what y ou would use though.
Anti- Ras would certainly handicap them but not sure of its lethality.
gmorley at rpms.ac.uk
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