isolation of PolyA+/A- RNA

Dr. Duncan Clark duncan at
Thu Jul 24 05:31:49 EST 1997

In article <Pine.A41.3.95b.970723140807.100058A-100000 at homer01.u.washing>, "N. Salazar" <nsalazar at U.WASHINGTON.EDU> writes
>I started off with 910 micrograms of total RNA, and got 14 micrograms of
>PolyA+ RNA, this represents about 1.6%. According to the information
>supplied with the kit, one is supposed to get about 2% of PolyA+ RNA.

Am I right in thinking that the yield of polyA+ will be organism
dependant? Anyway 1.6% isn't that far off 2%.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

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