GST-fusion protein coupling

Anton Andonov aandonov at
Thu Jul 24 11:12:07 EST 1997

I need a protocol to couple the GST-fusion protein to the gluthathione 
agarose support for affinity purification of antisera and optimal 
conditions for the elution of the bound antibody.
Any references or working in-house protocols will be highly appreciated.
Thank you.
Dr. Anton Andonov
Lab Centre for Disease Control
Ottawa, Ontario

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