Recycling Coomassie destain
drm21 at mole.bio.cam.ac.uk
Thu Jul 24 08:51:52 EST 1997
In article <8014181823071997.A07419.ATO4.11B7BC920D00*@MHS>,
M.W.T.WERTEN at ato.dlo.nl (WERTEN) wrote:
>In order to reduce the amount of chemical waste, our lab would like to recycle
>the MeOH/HAc destain we use for Coomassie stained gels, by removing the stain.
>Has anyone figured out a convenient way of doing this? Is activated carbon the
>best thing to use, or will filtration through a thick stack of paper do?
>Any suggestions are welcome,
>Marc Werten (m.w.t.werten at ato.dlo.nl)
>Dept. Microbial Conversions
>Agrotechnological Research Institute (ATO-DLO)
>6708 PD Wageningen
Foam bungs seem to pull out the blue dye pretty well - I used to drop one
in to speed up destaining.
An alternative is to use a colloidal coomassie stain (which is what I do
now). Its available from several people (Integrated Separation Systems
Pro-Blue and Sigma, at least) or you can make it yourself. Then you don't
need to destain, thought the constituents may be worse than MeOH/Acetic
acid: phosphoric acid, ammonium sulphate and MeOH
The original reference is:
Neuhoff V, Arold N, Taube D, Ehrhardt W: Improved staining of proteins in
polyacrylamide gels including isoelectric focusing gels with clear
background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and
R-250. Electrophoresis 1988, 9:255-262.
First heard of it here on methods and reagents....
D.R.Micklem, Time flies like an arrow...
Wellcome/CRC Institute, Fruit flies like a banana.
Cambridge CB2 1QR, UK Email:drm21 at mole.bio.cam.ac.uk
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