(no subject)

dicroot dicroot at GIASCL01.VSNL.NET.IN
Fri Jul 25 07:21:55 EST 1997


I had been trying to study short transcripts from an invitro
transcription experiment, and used a 25% PAGE. After the gel
was run, it was extremely difficult to get the gel to stick
to a Whatman 3MM in order to dry it. It's the first time that
I am handling a large, 0.4mm gel of this high acrylamide concn.
We have tried a couple of times, and invariably ended up
in a folded, torn gel that wouldn't stick to the paper, though
it comes off the glass plate during MeOH-AcOH fixing.

Any suggstions ? Please reply to pradeep at boseinst.ernet.in

Thank you.
Pradeep Parrack

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