GC-rich Sequencing

Catherine Burgess Catherine.Burgess at cmm.ki.se
Fri Jul 25 06:24:11 EST 1997

I am working with excessively GC-rich templates (to 86%) and am in GREAT
need of sequencing through these regions. I am using plasmid DNA with
the insert of interest (very clean) but my sequence (on ABI 377) fades
when it enters the higher GC-rich region (conveniently located after a
76% GC-rich flanking region next to CTG repeats). Can anyone suggest any
approaches which might be useful? I'm using 7-deaza-guanosine in the
sequencing reaction and hot-starts.

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