gel electrophoresis problem

dicroot dicroot at GIASCL01.VSNL.NET.IN
Mon Jul 28 01:29:44 EST 1997


This is my second attempt in two days in posting my problem
to this forum: I am facing a problem with 25% polyacrylamide
urea gel : After electrophoresis, the gel refuses to stick to
Whatmann 3MM paper. It folds or breaks. It comes off the glass
plate only when washed with MeOH-AcOH solution, but doesn't
come onto the paper. I do not have problems with lower percen-
tage gels (8%) of this sort. I haven't handled this high % gel
before. Seek help and advice from workers experienced in this.

pradeep at boseinst.ernet.in
July 28, 1997

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