Q: Semi-quantification

Eric Lader lader at ambion.com
Mon Jul 28 20:00:49 EST 1997


On 11 Jul 1997 08:17:58 -0700, zxz4 at PSU.EDU ("Z. Zhao") wrote:

>Dear Colleagues:
>
>The concept of semi-quantification has puzzled me for a long time. There 
>have been discussions on semi-quantitative PCR for many times in this 
>group. 

Semi Quantitative should read "relative quantitation' This means the
ability to compare expression between samples without determining
actual numbers of molecules. This is analagous to comparing bands on a
Northern blot. Without a standard curve, Northerns are only relative.

Get it?



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