Q: Semi-quantification

Eric Lader lader at ambion.com
Mon Jul 28 20:00:49 EST 1997

On 11 Jul 1997 08:17:58 -0700, zxz4 at PSU.EDU ("Z. Zhao") wrote:

>Dear Colleagues:
>The concept of semi-quantification has puzzled me for a long time. There 
>have been discussions on semi-quantitative PCR for many times in this 

Semi Quantitative should read "relative quantitation' This means the
ability to compare expression between samples without determining
actual numbers of molecules. This is analagous to comparing bands on a
Northern blot. Without a standard curve, Northerns are only relative.

Get it?

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