dirty RNA prep
cfb7 at psu.edu
cfb7 at psu.edu
Tue Jul 29 10:53:58 EST 1997
Hi,
I purified total RNA from mouse liver and spleen and while the spleen
RNA is clean and usable, the liver RNA is filthy. It has a greenish
-yellow tint and a 260/280 ratio of 1.02. My suspicion is that the
contaminating stuff is bile salts, but I don't know how to remove them.
Phenol:chloroform extraction didn't help. Any hints? Thanks,
Christie
Christie Blackman cfb7 at psu.edu
Department of Biochemistry
and Molecular Biology
Penn State University
University Park, PA
"There are alot of bastards in the world, the number increasing the
further one gets from Missoula, Montana."
Norman McLean, A River Runs Through It
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