M13 T7 polymerase

Peter Wang plw at mrc-lmb.cam.ac.uk
Tue Jul 29 19:31:16 EST 1997


Tim Motley wrote:
> I am looking for a M13 phage that carries the T7 polymerase. I have a T7
> construct of a lethal gene. I would like to grow it up and then infect
> with the M13-T7 phage to induce the gene.

Such a phage is available from Invitrogen with their pRSET Xpress 
expression kit.  I don't know if they sell it separately.

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Peter Wang, M.D., Ph.D.
MRC Centre for Protein Engineering,
Hills Road, Cambridge, CB2 2QH, England

Tel (01223) 402104  (international calls +44-1223-402104)
Fax (01223) 402140  (     "          "   +44-1223-402140)
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