dirty RNA prep
dariush at INAME.COM
Tue Jul 29 23:53:29 EST 1997
I've had similar problems with RNA extracted
from frozen liver sections. Seems that bile
pigments are ethanol soluble to some extent, yet
the final product is still green. I'a afaraid I
know of no solution. However, we recently started
using Omega Biotek's Total RNA Kit for tissues, and
whatever their secret, RNA always comes out clean.
Seems Omega has been a well kept secret for a while;
the spin columns work, plus they're half the price of
Qiagen's RNeasy kits. I now get enough RNA from 30
mg tissue to routinely do RPA's. And I think Omega
still gives away trial kits, so may want to give `em
a try. Their E-mail is info at omegabiotek.com. I can
get you the toll-free # if you want.
Good luck anyway.
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