PCR of Circular DNA

ma ddsyr at NS.EAST.CN.NET
Tue Jul 29 23:02:06 EST 1997

Dear PCR Colleagues:
Does anyone have the experiences about PCR the circular DNA like plasmid? I mean 
that when using two primers in PCR reaction, could we get two products? I met this 
situation now when I PCR a plasmid pGEM3Z, I got two bands, one is 700bp, another 
is 2 kb. I just wonder the reasons.

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