M13 T7 polymerase

bradley turner turner at SPRCORE.BIDMC.HARVARD.EDU
Tue Jul 29 20:11:06 EST 1997


The T7 polymerase is also available in a lambda phage CE6 available from
Novagen if you are interested, although I guess this would lyse your cells.
although it will induce expression when infected into a host carrying
an expression vector with a T7 promoter

Bacteriophage CE6 0.2ml cat. no. 69390-1 $50
                  10ml  cat. no. 69390-2 $200

800-207-0144 phone
608-238-1388 fax
novatech at novagen.com

Brad Turner (no affillation)

  		    Bradley Turner
                Beth Israel Deaconess
                    Medical Center

Harvard Medical School		617-667-1215 phone
Division of Gastroenterology	617-667-2767 fax
Room Dana 536			bsturner at mbcrr.harvard.edu
330 Brookline Avenue		bturner at bidmc.harvard.edu
Boston, MA 02215		turner at sprcore.bidmc.harvard.edu

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To: methods at net.bio.net
From: Peter Wang <plw at mrc-lmb.cam.ac.uk>
Subject: Re: M13 T7 polymerase
Date: Wed, 30 Jul 1997 00:31:16 +0000
Message-Id: <33DE8B54.3525 at mrc-lmb.cam.ac.uk>
To: Tim Motley <tmotley at u.washington.edu>
Status: R

Tim Motley wrote:
> I am looking for a M13 phage that carries the T7 polymerase. I have a T7
> construct of a lethal gene. I would like to grow it up and then infect
> with the M13-T7 phage to induce the gene.

Such a phage is available from Invitrogen with their pRSET Xpress 
expression kit.  I don't know if they sell it separately.

Peter Wang, M.D., Ph.D.
MRC Centre for Protein Engineering,
Hills Road, Cambridge, CB2 2QH, England

Tel (01223) 402104  (international calls +44-1223-402104)
Fax (01223) 402140  (     "          "   +44-1223-402140)

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