High Molecular Weight DNA
maieru at uni-muenster.de
Wed Jul 30 08:51:09 EST 1997
Shou-hsien Li <sl7599 at cnsibm.albany.edu> wrote:
>I am considering trying Qiagen's Genomic tips DNA extraction kit to
>obtain relatively high molecular wt. DNA from frozen tissue for lambda
>cloning and southerns. Does anyone have any experience with this kit?
>Did you like it; was the DNA size about 100kb or better and was the
>DNA obtained clean? Thanks!
I have worked two or three years ago with the genomic DNA isolation
kit from QIAgen based on the Tip 20G/100G columns.
The results were consistend with earlier experiences with QIAgen. The
eluat from the column is suitable for every follow-up step, for
example digestion with critical REs, the yield was ~20 percent of
classic isolation methods.
(I am getting NO money from QIAgen!)
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