Help ! RNA prep from less than 1 million cells

Aki Hoji akhst7 at pop.pitt.edu
Wed Jul 30 18:00:17 EST 1997


Hi all, 

I have been trying to extract as much total RNA from less than 1 million
PBMC and from frozen PBMC as I could but my yield never has reached the
optimal amount which is 10ug.  I've tried several different methods and
tricks but the most that I've got is around 6ug.  If you have any tricks
or kits that I cay try out, please, let me know.  Thanks in advance !!

Aki



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