PCR Using T7 and SP6 Primers

ma ddsyr at NS.EAST.CN.NET
Wed Jul 30 21:24:47 EST 1997


Dear Netters:
I want to check the insert in plasmid (pGEM), so I plan to use a universe primers, 
say T7(5'AAT ACG ACT CAC TAT AG 3') and SP6(5' ATT TAG GTG ACA CTA TAG 3'), which 
are also used in DNA sequence. But I find the Tm of these two primers are very low, 
only 42-45. So I guess this may influence these PCR efficiency. Hope the experts 
give me their experiences and suggestions. Thanks
Ma




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